{"id":11243,"date":"2022-11-21T05:08:15","date_gmt":"2022-11-20T21:08:15","guid":{"rendered":"https:\/\/www.pyrazoles-derivatives.com\/?p=11243"},"modified":"2022-11-21T05:08:15","modified_gmt":"2022-11-20T21:08:15","slug":"kapara-anastasias-team-published-research-in-analytical-chemistry-washington-dc-united-states-in-93-cas-19959-71-8","status":"publish","type":"post","link":"https:\/\/www.pyrazoles-derivatives.com\/?p=11243","title":{"rendered":"Kapara, Anastasia’s team published research in Analytical Chemistry (Washington, DC, United States) in 93 | CAS: 19959-71-8"},"content":{"rendered":"

Kapara, Anastasia published the artcileDetection of Estrogen Receptor Alpha and Assessment of Fulvestrant Activity in MCF-7 Tumor Spheroids Using Microfluidics and SERS<\/i><\/b>, Quality Control of 19959-71-8<\/a>, the publication is Analytical Chemistry (Washington, DC, United States) (2021), 93(14), 5862-5871, database is CAplus and MEDLINE.<\/p>\n

Breast cancer is one of the leading causes of cancer death in women. Novel in vitro tools that integrate three-dimensional (3D) tumor models with highly sensitive chem. reporters can provide useful information to aid biol. characterization of cancer phenotype and understanding of drug activity. The combination of surface-enhanced Raman scattering (SERS) techniques with microfluidic technologies offers new opportunities for highly selective, specific, and multiplexed nanoparticle-based assays. Here, we explored the use of functionalized nanoparticles for the detection of estrogen receptor alpha (ER\u03b1) expression in a 3D tumor model, using the ER\u03b1-pos. human breast cancer cell line MCF-7. This approach was used to compare targeted vs. nontargeted nanoparticle interactions with the tumor model to better understand whether targeted nanotags are required to efficiently target ER\u03b1. Mixtures of targeted anti-ER\u03b1 antibody-functionalized nanotags (ER\u03b1-AuNPs) and nontargeted (against ER\u03b1) anti-human epidermal growth factor receptor 2 (HER2) antibody-functionalized nanotags (HER2-AuNPs), with different Raman reporters with a similar SERS signal intensity, were incubated with MCF-7 spheroids in microfluidic devices and spectroscopically analyzed using SERS. MCF-7 cells expressed high levels of ER\u03b1 and no detectable levels of HER2. 2D and 3D SERS measurements confirmed the strong targeting effect of ER\u03b1-AuNP nanotags to the MCF-7 spheroids in contrast to HER2-AuNPs (63% signal reduction). Moreover, 3D SERS measurements confirmed the differentiation between the targeted and the nontargeted nanotags. Finally, we demonstrated how the nanotag uptake on MCF-7 spheroids was affected by the drug fulvestrant, the first-in-class approved selective estrogen receptor degrader (SERD). These results illustrate the potential of using SERS and microfluidics as a powerful in vitro platform for the characterization of 3D tumor models and the investigation of SERD activity.<\/p>\n

Analytical Chemistry (Washington, DC, United States) published new progress about 19959-71-8. 19959-71-8 belongs to pyrazoles-derivatives, auxiliary class Pyrazole,Pyridine, name is 4-(1H-Pyrazol-4-yl)pyridine, and the molecular formula is C8H7N3, Quality Control of 19959-71-8<\/a>.<\/p>\n

Referemce:
https:\/\/en.wikipedia.org\/wiki\/Pyrazole<\/a>,
Pyrazoles – an overview | ScienceDirect Topics<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Analytical Chemistry (Washington, DC, United States) published new progress about 19959-71-8. 19959-71-8 belongs to pyrazoles-derivatives, auxiliary class Pyrazole,Pyridine, name is 4-(1H-Pyrazol-4-yl)pyridine, and the molecular formula is C8H7N3, Quality Control of 19959-71-8<\/a>.<\/p>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[131],"tags":[732],"class_list":["post-11243","post","type-post","status-publish","format-standard","hentry","category-pyrazoles-derivatives","tag-m-w100-150"],"yoast_head":"\nKapara, Anastasia's team published research in Analytical Chemistry (Washington, DC, United States) in 93 | CAS: 19959-71-8 | pyrazoles-derivatives<\/title>\n<meta name=\"description\" content=\"Kapara, Anastasia published the artcileDetection of Estrogen Receptor Alpha and Assessment of Fulvestrant Activity in MCF-7 Tumor Spheroids Using Microfluidics and SERS, Quality Control of 19959-71-8, the publication is Analytical Chemistry (Washington, DC, United States) (2021), 93(14), 5862-5871, database is CAplus and MEDLINE.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.pyrazoles-derivatives.com\/?p=11243\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Kapara, Anastasia's team published research in Analytical Chemistry (Washington, DC, United States) in 93 | CAS: 19959-71-8 | pyrazoles-derivatives\" \/>\n<meta property=\"og:description\" content=\"Kapara, Anastasia published the artcileDetection of Estrogen Receptor Alpha and Assessment of Fulvestrant Activity in MCF-7 Tumor Spheroids Using Microfluidics and SERS, Quality Control of 19959-71-8, the publication is Analytical Chemistry (Washington, DC, United States) (2021), 93(14), 5862-5871, database is CAplus and MEDLINE.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.pyrazoles-derivatives.com\/?p=11243\" \/>\n<meta property=\"og:site_name\" content=\"pyrazoles-derivatives\" \/>\n<meta property=\"article:published_time\" content=\"2022-11-20T21:08:15+00:00\" \/>\n<meta name=\"author\" content=\"Jessica.F\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Jessica.F\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.pyrazoles-derivatives.com\/?p=11243\",\"url\":\"https:\/\/www.pyrazoles-derivatives.com\/?p=11243\",\"name\":\"Kapara, Anastasia's team published research in Analytical Chemistry (Washington, DC, United States) in 93 | CAS: 19959-71-8 | pyrazoles-derivatives\",\"isPartOf\":{\"@id\":\"https:\/\/www.pyrazoles-derivatives.com\/#website\"},\"datePublished\":\"2022-11-20T21:08:15+00:00\",\"author\":{\"@id\":\"https:\/\/www.pyrazoles-derivatives.com\/#\/schema\/person\/d9a9f27bed675392c6f363a01ffd49f1\"},\"description\":\"Kapara, Anastasia published the artcileDetection of Estrogen Receptor Alpha and Assessment of Fulvestrant Activity in MCF-7 Tumor Spheroids Using Microfluidics and SERS, Quality Control of 19959-71-8, the publication is Analytical Chemistry (Washington, DC, United States) (2021), 93(14), 5862-5871, database is CAplus and MEDLINE.\",\"breadcrumb\":{\"@id\":\"https:\/\/www.pyrazoles-derivatives.com\/?p=11243#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.pyrazoles-derivatives.com\/?p=11243\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.pyrazoles-derivatives.com\/?p=11243#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"\u9996\u9875\",\"item\":\"https:\/\/www.pyrazoles-derivatives.com\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Kapara, Anastasia’s team published research in Analytical Chemistry (Washington, DC, United States) in 93 | CAS: 19959-71-8\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.pyrazoles-derivatives.com\/#website\",\"url\":\"https:\/\/www.pyrazoles-derivatives.com\/\",\"name\":\"pyrazoles-derivatives\",\"description\":\"Several pyrazole derivatives possess important pharmacological activities and they have been proved useful materials in drug research. 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